2ZBM

Crystal Structure of I115M Mutant Cold-Active Protein Tyrosine Phosphatase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	103
Detector technology	CCD
Collection date	2005-05-16
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	54.172, 77.559, 80.331
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.1216
R-free	0.19730
Structure solution method	AB INITIO PHASING
RMSD bond length	0.012
RMSD bond angle	0.031
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.550
High resolution limit [Å]	1.500	1.500
Number of reflections	54976
Completeness [%]	97.7	84.7
Redundancy	9.1	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	15% POLYETHYLENEGLYCOL 4000, 0.1M AMMONIUM ACETATE, 0.05M TRIS-HCL (PH 8.5), VAPOR DIFFUSION, HANGING DROP, temperature 277K