2ZBG
Calcium pump crystal structure with bound AlF4 and TG in the absence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 177.500, 70.200, 143.400 |
Unit cell angles | 90.00, 106.80, 90.00 |
Refinement procedure
Resolution | 11.990 - 2.550 |
R-factor | 0.224 |
Rwork | 0.224 |
R-free | 0.24800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wpg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.200 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.550 | 2.550 |
Rmerge | 0.057 | 0.286 |
Number of reflections | 55231 | |
<I/σ(I)> | 32.8 | 2 |
Completeness [%] | 99.8 | 96.3 |
Redundancy | 7.2 | 3.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 6.1 | 283 | pH 6.1, MICRODIALYSIS, temperature 283K |