2ZBF
Calcium pump crystal structure with bound BeF3 and TG in the absence of calcium
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-17 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 90.522, 136.473, 106.619 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 11.980 - 2.400 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.25700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wpg |
RMSD bond length | 0.007 |
RMSD bond angle | 1.300 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.450 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.085 | 0.279 |
Number of reflections | 50077 | |
<I/σ(I)> | 17 | 2.2 |
Completeness [%] | 95.5 | 79.9 |
Redundancy | 6.8 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | MICRODIALYSIS | 5.7 | 283 | pH 5.7, MICRODIALYSIS, temperature 283K |