2ZBE
Calcium pump crystal structure with bound BeF3 in the absence of calcium and TG
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-10-14 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 133.869, 91.592, 248.464 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 12.000 - 3.800 |
| R-factor | 0.293 |
| Rwork | 0.293 |
| R-free | 0.32700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zbf |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.600 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.940 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.094 | |
| Number of reflections | 30237 | |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 99.2 | 99.6 |
| Redundancy | 4.4 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 5.7 | 283 | pH 5.7, MICRODIALYSIS, temperature 283K |
