2ZBD
Crystal Structure of the SR Calcium Pump with Bound Aluminium Fluoride, ADP and Calcium
Replaces: 2Z9RReplaces: 1WPEExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-07-13 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 162.900, 75.100, 152.100 |
| Unit cell angles | 90.00, 109.30, 90.00 |
Refinement procedure
| Resolution | 12.000 - 2.400 |
| R-factor | 0.24759 |
| Rwork | 0.246 |
| R-free | 0.27136 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1WPE |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.010 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.054 | 0.350 |
| Number of reflections | 61807 | |
| <I/σ(I)> | 27.7 | 2.7 |
| Completeness [%] | 90.8 | 58.5 |
| Redundancy | 3.5 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.1 | 283 | PEG 400, pH 6.10, MICRODIALYSIS, temperature 283K |
