2ZBC
Crystal structure of STS042, a stand-alone RAM module protein, from hyperthermophilic archaeon Sulfolobus tokodaii strain7.
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9792, 0.9793, 0.9641 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 61.367, 61.785, 90.487 |
| Unit cell angles | 90.00, 94.47, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.900 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21900 |
| Structure solution method | MAD |
| RMSD bond length | 0.027 |
| RMSD bond angle | 2.081 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.000 | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.080 | 1.900 |
| Rmerge | 0.104 | 0.069 | 0.244 |
| Number of reflections | 52841 | ||
| <I/σ(I)> | 9.3 | ||
| Completeness [%] | 98.6 | 98.1 | 96.6 |
| Redundancy | 3.3 | 3.4 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | VAPOR DIFFUSION, SITTING DROP |
