2ZB2
Human liver glycogen phosphorylase a complexed with glcose and 5-chloro-N-[4-(1,2-dihydroxyethyl)phenyl]-1H-indole-2-carboxamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6B |
| Synchrotron site | Photon Factory |
| Beamline | BL-6B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-10-18 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 |
| Unit cell lengths | 123.819, 123.819, 123.538 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.180 - 2.450 |
| R-factor | 0.3 |
| Rwork | 0.261 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1exv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.300 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 2.490 |
| High resolution limit [Å] | 2.420 | 2.420 |
| Rmerge | 0.300 | |
| Number of reflections | 79745 | |
| <I/σ(I)> | 1.4 | 1.8 |
| Completeness [%] | 99.1 | 91.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 100mM NaMES (pH6), 22.5 % MPD, 60mM D-glucose, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
