2ZAY

Crystal structure of response regulator from Desulfuromonas acetoxidans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-09-15
Detector	ADSC QUANTUM 4
Wavelength(s)	0.97915
Spacegroup name	P 21 21 21
Unit cell lengths	47.215, 59.016, 88.967
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.590 - 2.000
R-factor	0.225
Rwork	0.225
R-free	0.26100
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	25.000
High resolution limit [Å]	2.000
Rmerge	0.083
Number of reflections	17384
Completeness [%]	99.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	277	3.5 M Sodium formate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K