2ZAU
Crystal structure of an N-terminally truncated selenophosphate synthetase from Aquifex aeolicus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-25 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 93.161, 165.198, 167.684 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.290 - 2.000 |
R-factor | 0.212 |
Rwork | 0.212 |
R-free | 0.23900 |
Structure solution method | MIR |
RMSD bond length | 0.154 |
RMSD bond angle | 1.700 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.097 | 0.429 |
Number of reflections | 82314 | |
<I/σ(I)> | 18.3 | 3.66 |
Completeness [%] | 93.9 | 94.7 |
Redundancy | 4.3 | 3.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.1 | 293 | MPD, AMMONIUM PHOSPHATE, NA-HEPES, pH 7.10, VAPOR DIFFUSION, SITTING DROP, temperature 293K |