2ZAI
Crystal structure of the soluble domain of STT3 from P. furiosus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-05 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.96419 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 136.935, 265.297, 74.138 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.227 |
| R-free | 0.27270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zag |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.081 | 0.443 |
| Number of reflections | 75226 | |
| <I/σ(I)> | 12.4 | 3.5 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.1 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 303 | 22% PEG 4000, 0.3M NaCOOH, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 303K |
