2ZAH
X-ray structure of Melon necrotic spot virus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | MACSCIENCE |
| Wavelength(s) | 0.900 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 375.004, 375.004, 375.004 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 187.500 - 2.810 |
| R-factor | 0.20916 |
| Rwork | 0.208 |
| R-free | 0.22373 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2tbv |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | DM |
| Refinement software | REFMAC (5.3.0022) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 267.000 | 2.940 |
| High resolution limit [Å] | 2.790 | 2.790 |
| Rmerge | 0.164 | 0.608 |
| Number of reflections | 208594 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 97.0 | |
| Redundancy | 16.4 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 277 | PEG2000, phosphate buffer, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
