2ZA8
recombinant horse L-chain apoferritin N-terminal deletion mutant (residues 1-8)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 180.210, 180.210, 180.210 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.760 - 1.400 |
R-factor | 0.22066 |
Rwork | 0.220 |
R-free | 0.23855 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aew |
RMSD bond length | 0.008 |
RMSD bond angle | 1.111 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 1.400 |
Number of reflections | 52831 |
Completeness [%] | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.8 | 293 | 0.1M Tris-HCl, 225mM ammonium sulphate, 0.13% cadmium chloride, 25% 2-Metyl-2,4-pentanediol, pH 6.8, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |