2ZA7
recombinant horse L-chain apoferritin N-terminal deletion mutant (residues 1-4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Detector technology | CCD |
Collection date | 2003-10-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9 |
Spacegroup name | F 4 3 2 |
Unit cell lengths | 180.280, 180.280, 180.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.520 - 1.400 |
R-factor | 0.22285 |
Rwork | 0.222 |
R-free | 0.24237 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1aew |
RMSD bond length | 0.011 |
RMSD bond angle | 1.204 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.1.19) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 41.520 |
High resolution limit [Å] | 1.400 |
Number of reflections | 51287 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 0.1M Tris-HCl, 0.13% cadmium chloride, 15% PEG4000, 190mM calcium chloride, 5% glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |