2ZA1
Crystal Structure of orotidine 5'-monophosphate decarboxylase complexed with orotidine 5'-monophosphate from P.falciparum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2006-11-18 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | H 3 |
Unit cell lengths | 201.800, 201.800, 44.200 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.140 - 2.650 |
R-factor | 0.21 |
Rwork | 0.210 |
R-free | 0.29200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2za2 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.800 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | AMoRE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.339 | |
Number of reflections | 20677 | |
Completeness [%] | 98.8 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.5 | 293 | 23% PEG 3000, pH 9.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |