2ZA0
Crystal structure of mouse glyoxalase I complexed with methyl-gerfelin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-05 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.7 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.982, 65.314, 66.159 |
| Unit cell angles | 90.00, 101.18, 90.00 |
Refinement procedure
| Resolution | 23.380 - 1.700 |
| Rwork | 0.185 |
| R-free | 0.21500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qip |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.760 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.068 | 0.301 |
| Number of reflections | 36563 | |
| <I/σ(I)> | 17.53 | 3.15 |
| Completeness [%] | 94.4 | 100 |
| Redundancy | 3.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.8 | 293 | PEG2000mme, MES, NaCl, pH5.8, VAPOR DIFFUSION, temperature 293K |
