2Z9Z
Crystal structure of CERT START domain(N504A mutant), in complex with C10-diacylglycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2007-03-02 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.768, 75.374, 42.439 |
| Unit cell angles | 90.00, 108.85, 90.00 |
Refinement procedure
| Resolution | 40.160 - 1.740 |
| R-factor | 0.24178 |
| Rwork | 0.240 |
| R-free | 0.27951 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2e3n |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.402 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.800 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Number of reflections | 23708 | |
| <I/σ(I)> | 48.7 | 1.8 |
| Completeness [%] | 95.2 | 70.9 |
| Redundancy | 4.8 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | VAPOR DIFFUSION, HANGING DROP |
