2Z9Z

Crystal structure of CERT START domain(N504A mutant), in complex with C10-diacylglycerol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	95
Detector technology	CCD
Collection date	2007-03-02
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 1 21 1
Unit cell lengths	40.768, 75.374, 42.439
Unit cell angles	90.00, 108.85, 90.00

Refinement procedure

Resolution	40.160 - 1.740
R-factor	0.24178
Rwork	0.240
R-free	0.27951
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2e3n
RMSD bond length	0.012
RMSD bond angle	1.402
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.800
High resolution limit [Å]	1.740	1.740
Number of reflections	23708
<I/σ(I)>	48.7	1.8
Completeness [%]	95.2	70.9
Redundancy	4.8	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP			VAPOR DIFFUSION, HANGING DROP