2Z9T
Crystal structure of the human beta-2 microglobulin mutant W60G
Experimental procedure
| Experimental method | LAUE |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-02-06 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.873 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 77.678, 29.014, 59.160 |
| Unit cell angles | 90.00, 125.26, 90.00 |
Refinement procedure
| Resolution | 19.160 - 1.800 |
| R-factor | 0.17594 |
| Rwork | 0.174 |
| R-free | 0.21424 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1py4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.241 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.200 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Number of reflections | 9273 | |
| <I/σ(I)> | 14.9 | 8 |
| Completeness [%] | 93.2 | 95.9 |
| Redundancy | 2.9 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 0.1M MES, PEG 4000, Ammonium acetate, glycerol, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
