2Z91

Crystal structure of the Fab fragment of anti-ciguatoxin antibody 10C9

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NW12A
Synchrotron site	Photon Factory
Beamline	AR-NW12A
Temperature [K]	100
Detector technology	CCD
Collection date	2007-02-28
Detector	ADSC QUANTUM 210
Wavelength(s)	1.0000
Spacegroup name	P 1 21 1
Unit cell lengths	67.844, 78.263, 89.042
Unit cell angles	90.00, 99.25, 90.00

Refinement procedure

Resolution	10.000 - 2.600
R-factor	0.22
Rwork	0.220
R-free	0.27000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z92
RMSD bond length	0.008
RMSD bond angle	1.600
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.690
High resolution limit [Å]	2.600	2.600
Number of reflections	28425
Redundancy	3.8	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.2	293	0.2M Zinc Acetate, 0.1M Sodium Cacodylate, 15% PEG 8000, pH5.2, VAPOR DIFFUSION, HANGING DROP, temperature 293K