2Z90

Crystal Structure of the Second Dps from Mycobacterium smegmatis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU ULTRAX 18
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2006-05-24
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.5418
Spacegroup name	H 3 2
Unit cell lengths	90.008, 90.008, 421.522
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.940 - 2.400
R-factor	0.187
Rwork	0.187
R-free	0.22700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o9r
RMSD bond length	0.006
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.490
High resolution limit [Å]	2.400	2.400
Number of reflections	26434
<I/σ(I)>	15.3	3.5
Completeness [%]	99.9	99.3
Redundancy	17.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microbatch method under oil	6.5	293	20mM Tris-HCl (pH 7.9), 200 mM NaCl, PEG-3000, 200mM MgCl2, 0.1M sodium cacodylate (pH 6.5), Microbatch method under oil, temperature 293K