2Z8Z
Crystal structure of a platinum-bound S445C mutant of Pseudomonas sp. MIS38 lipase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL38B1 |
Synchrotron site | SPring-8 |
Beamline | BL38B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-05-19 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.071784 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.879, 84.404, 87.012 |
Unit cell angles | 90.00, 96.50, 90.00 |
Refinement procedure
Resolution | 32.120 - 1.800 |
R-factor | 0.17512 |
Rwork | 0.174 |
R-free | 0.20493 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z8x |
RMSD bond length | 0.020 |
RMSD bond angle | 1.771 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 1.800 |
Rmerge | 0.074 |
Number of reflections | 63435 |
Redundancy | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 277 | 20% PEG 20000, 0.1M MES, 0.2M calcium acetate, 5mM zinc acetate, pH6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |