2Z8W
Structure of an IgNAR-AMA1 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-18B |
Synchrotron site | Photon Factory |
Beamline | BL-18B |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-21 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96426 |
Spacegroup name | P 31 |
Unit cell lengths | 76.480, 76.480, 140.978 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 38.330 - 2.450 |
R-factor | 0.19822 |
Rwork | 0.189 |
R-free | 0.28183 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1Z40 and 1VER |
RMSD bond length | 0.011 |
RMSD bond angle | 1.340 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.230 | 2.510 |
High resolution limit [Å] | 2.450 | 2.450 |
Rmerge | 0.130 | 0.570 |
Number of reflections | 28622 | |
<I/σ(I)> | 14.4 | 1.6 |
Redundancy | 5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.2 | 298 | 0.1M phosphate citrate pH 4.2, 0.2M NaCl, 20% PEG 8000, VAPOR DIFFUSION, HANGING DROP, temperature 298K |