2Z8N
Structural basis for the catalytic mechanism of phosphothreonine lyase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2006-12-26 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.030, 54.920, 90.730 |
| Unit cell angles | 90.00, 92.61, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.800 |
| Rwork | 0.212 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z8o |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.678 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.040 | 0.175 |
| Number of reflections | 38881 | |
| <I/σ(I)> | 30.66 | 9.8 |
| Completeness [%] | 98.8 | 97.1 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350, 100mM Tris, 0.2mM ammonium sulfate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
