2Z8M
Structural basis for the catalytic mechanism of phosphothreonine lyase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU300 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2006-12-16 |
Detector | RIGAKU RAXIS IV |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.490, 71.950, 106.270 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 2.000 |
Rwork | 0.205 |
R-free | 0.23000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z8o |
RMSD bond length | 0.006 |
RMSD bond angle | 1.210 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SOLVE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 99.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.055 | 0.298 |
Number of reflections | 32847 | |
<I/σ(I)> | 36.3 | 7.9 |
Completeness [%] | 96.4 | 94.7 |
Redundancy | 7.9 | 7.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 4000, 100mM HEPES, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |