2Z8K
Crystal Structure of Escherichia coli gamma-Glutamyltranspeptidase in Complex with Acivicin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-06-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 77.730, 126.500, 129.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.160 - 1.650 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.21500 |
Structure solution method | 2DBU |
RMSD bond length | 0.004 |
RMSD bond angle | 1.300 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.710 |
High resolution limit [Å] | 1.650 | 1.650 |
Rmerge | 0.046 | 0.211 |
Number of reflections | 148397 | |
<I/σ(I)> | 18.7 | |
Completeness [%] | 96.8 | 90.6 |
Redundancy | 4.7 | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.4 | 277 | 12.5-17.5% PEG 4000, 0.2M CaCl2, 0.1M Tris-HCl, pH 8.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |