2Z83
Crystal Structure of Catalytic Domain of Japanese Encephalitis Virus NS3 Helicase/Nucleoside Triphosphatase at a Resolution 1.8
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-10-26 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.062, 68.024, 65.219 |
| Unit cell angles | 90.00, 116.87, 90.00 |
Refinement procedure
| Resolution | 32.410 - 1.800 |
| R-factor | 0.20196 |
| Rwork | 0.199 |
| R-free | 0.24918 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bhr |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.592 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 32.410 |
| High resolution limit [Å] | 1.800 |
| Number of reflections | 40575 |
| Completeness [%] | 99.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 15 % ethanol, 100mM Tris-HCl pH 7.0, 4% pentaerythritol etoxylate (3/4 EO/OH), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
