2Z7W
Crystal Structure of H2O2 treated Cu,Zn-SOD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-5A |
Synchrotron site | Photon Factory |
Beamline | BL-5A |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2007-06-03 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.000000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.085, 50.714, 149.341 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.070 - 1.800 |
Rwork | 0.210 |
R-free | 0.23400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1e9q |
RMSD bond length | 0.005 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.070 |
High resolution limit [Å] | 1.800 |
Number of reflections | 32640 |
Completeness [%] | 94.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.4 | 295 | 20% (w/v) PEG 4000, 20% (w/v) iso-propanol, 0.09M MES, 1mM EDTA, pH 6.4-6.7, VAPOR DIFFUSION, SITTING DROP, temperature 295K |