2Z7J

Structural insights into de multifunctional VP3 protein of birnaviruses:gold derivative

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-2
Synchrotron site	ESRF
Beamline	ID23-2
Temperature [K]	100
Detector technology	CCD
Collection date	2006-03-04
Detector	ADSC QUANTUM 315
Wavelength(s)	1.039 1.040 1.037
Spacegroup name	P 41 21 2
Unit cell lengths	69.749, 69.749, 71.656
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.400
R-factor	0.23834
Rwork	0.236
R-free	0.28492
RMSD bond length	0.008
RMSD bond angle	1.051
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.300	2.300
Number of reflections	7312
Completeness [%]	99.6	99.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5	298	2.5M NaCl 0.1M Sodium Acetate pH 5, VAPOR DIFFUSION, HANGING DROP, temperature 298K