2Z7G
Crystal structure of adenosine deaminase ligated with EHNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-07-31 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 77.100, 77.100, 135.660 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 67.030 - 2.520 |
| R-factor | 0.21 |
| Rwork | 0.207 |
| R-free | 0.24700 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1krm |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.600 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | MOLREP |
| Refinement software | CNX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.030 | 2.750 |
| High resolution limit [Å] | 2.520 | 2.520 |
| Rmerge | 0.062 | 0.167 |
| Number of reflections | 13675 | |
| <I/σ(I)> | 13.4 | 3.7 |
| Completeness [%] | 94.7 | 94.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 2M Ammonium sulfate, 2% MPD, 0.1M MES buffer, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
