2Z7C

Crystal structure of chromatin protein alba from hyperthermophilic archaeon pyrococcus horikoshii

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	100
Detector technology	CCD
Collection date	2007-04-16
Detector	RIGAKU JUPITER 210
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	41.262, 51.974, 173.004
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.550 - 2.800
R-factor	0.23
Rwork	0.230
R-free	0.31800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1y9x
RMSD bond length	0.007
RMSD bond angle	1.300
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	CNS (1.2)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.900
High resolution limit [Å]	2.790	2.800
Number of reflections	8238
Completeness [%]	83.1	51
Redundancy	5.1	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	9	293	15% PEGMME 550, 0.2M Sodium chloride, 0.1M Bicine, pH 9.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K