2Z72

New Structure Of Cold-Active Protein Tyrosine Phosphatase At 1.1 Angstrom

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL38B1
Synchrotron site	SPring-8
Beamline	BL38B1
Temperature [K]	103
Detector technology	CCD
Collection date	2005-11-11
Detector	RIGAKU JUPITER 210
Wavelength(s)	0.7
Spacegroup name	P 21 21 21
Unit cell lengths	55.565, 77.306, 81.029
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 1.100
R-factor	0.116
R-free	0.16120
Structure solution method	AB INITIO
Starting model (for MR)	1v73
RMSD bond length	0.017
RMSD bond angle	0.033
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.100
Number of reflections	141407
Completeness [%]	89.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	277	15% polyethyleneglycol, 0.1M ammonium acetate, 50mM p-nitrophenylsulphate, 0.05M Tris-HCl (pH8.5), pH8.50, VAPOR DIFFUSION, HANGING DROP, temperature 277.0K