2Z70
E.coli RNase 1 in complex with d(CGCGATCGCG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | X11 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-10-15 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.8125 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 35.556, 49.758, 143.108 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 1.700 |
R-factor | 0.19363 |
Rwork | 0.190 |
R-free | 0.23550 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2pqx |
RMSD bond length | 0.012 |
RMSD bond angle | 1.357 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.760 |
High resolution limit [Å] | 1.700 | 1.700 |
Rmerge | 0.046 | 0.319 |
Number of reflections | 28734 | |
<I/σ(I)> | 16.9 | 4.2 |
Completeness [%] | 99.6 | 99.9 |
Redundancy | 4.34 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 20% PEG8000, 0.1M Sodium acetate, 1mM MgCl2, 0.1M MES, pH6.1, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG8000 | ||
10 | 1 | 2 | HOH | ||
2 | 1 | 1 | Sodium acetate | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | MES | ||
5 | 1 | 1 | HOH | ||
6 | 1 | 2 | PEG8000 | ||
7 | 1 | 2 | Sodium acetate | ||
8 | 1 | 2 | MgCl2 | ||
9 | 1 | 2 | MES |