2Z6Y
Crystal structure of a photoswitchable GFP-like protein Dronpa in the bright-state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B1 |
Synchrotron site | SPring-8 |
Beamline | BL26B1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-03-27 |
Detector | RIGAKU JUPITER 210 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.889, 95.829, 74.011 |
Unit cell angles | 90.00, 115.62, 90.00 |
Refinement procedure
Resolution | 19.510 - 2.000 |
R-factor | 0.208 |
Rwork | 0.208 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2z6z |
RMSD bond length | 0.007 |
RMSD bond angle | 1.500 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.118 | 0.371 |
Number of reflections | 53536 | |
<I/σ(I)> | 4.1 | |
Completeness [%] | 99.3 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 20% PEG 3000, 0.2M Ca(acetate)2, 0.1M Tris/HCl (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 291K |