2Z6P
Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-23 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.900 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 46.829, 46.829, 143.338 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.500 - 1.800 |
R-factor | 0.19976 |
Rwork | 0.198 |
R-free | 0.24316 |
Structure solution method | SAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.344 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.500 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.081 | 0.332 |
Number of reflections | 15648 | |
<I/σ(I)> | 20.3 | |
Completeness [%] | 99.9 | 99.9 |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 1.5M ammonium sulfate, 0.1M MES pH 7.0, 10%(w/v) dioxan, VAPOR DIFFUSION, HANGING DROP, temperature 293K |