2Z6O
Crystal Structure of the Ufc1, Ufm1 conjugating enzyme 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-12-06 |
| Detector | Bruker DIP-6040 |
| Wavelength(s) | 0.9000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.370, 47.601, 82.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.200 - 1.600 |
| R-factor | 0.1956 |
| Rwork | 0.194 |
| R-free | 0.22125 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z6p |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.145 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.204 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.054 | 0.331 |
| Number of reflections | 22178 | |
| <I/σ(I)> | 16.8 | |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 28% (w/v) PEG 4000, 0.1M Tris-HCl pH 8.5, 0.2M MgCl2 , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
