2Z6N
Crystal Structure of Carbonmonoxy Hemoglobin D from the Aldabra Giant Tortoise, Geochelone gigantea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU ULTRAX 18 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-06-08 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 112.355, 62.104, 53.987 |
| Unit cell angles | 90.00, 110.22, 90.00 |
Refinement procedure
| Resolution | 30.570 - 1.860 |
| Rwork | 0.185 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1v75 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.000 |
| Data reduction software | CrystalClear |
| Data scaling software | CrystalClear |
| Phasing software | CNX |
| Refinement software | CNX (2005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.510 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.035 | 0.138 |
| Number of reflections | 28249 | |
| Completeness [%] | 96.0 | 95.4 |
| Redundancy | 3.76 | 3.74 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 7% PEG 3350, 50mM HEPES-Na, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
