2Z6M
Crystal structure of Human Ferritin H8 as biotemplate for noble metal nanoparticle synthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-07-28 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.080 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 171.294, 171.294, 190.455 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.720 |
| Rwork | 0.215 |
| R-free | 0.25200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fha |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.820 |
| High resolution limit [Å] | 2.720 | 2.720 |
| Rmerge | 0.096 | 0.400 |
| Number of reflections | 75646 | |
| <I/σ(I)> | 8.8 | 1.9 |
| Completeness [%] | 99.0 | 98.7 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 298 | protein solution(11.0 mg/mL H8 in unbuffered 3.0mM NaN3), 2.5mL of precipitant buffer(0.1 M sodium acetate(pH 4.6), 20%(v/v) isopropanol, 0.2M CaCl2) , VAPOR DIFFUSION, HANGING DROP, temperature 298K |
