2Z6H

Crystal Structure of Beta-Catenin Armadillo Repeat Region and Its C-Terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	170
Detector technology	CCD
Detector	CUSTOM-MADE
Wavelength(s)	1.00723, 1.07156
Spacegroup name	I 4 2 2
Unit cell lengths	104.229, 104.229, 259.391
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.690 - 2.200
R-factor	0.213
Rwork	0.213
R-free	0.24900
Structure solution method	SIRAS
RMSD bond length	0.006
RMSD bond angle	1.100
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.075	0.611
Number of reflections	29790
<I/σ(I)>	21.8	1.9
Completeness [%]	81.0	81.2
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	298	0.1M Na Citrate, 0.4M NaCl, 7% JEFFAMINE ED-2001, pH 5.60, VAPOR DIFFUSION, HANGING DROP, temperature 298K