2Z6E
Crystal Structure of Human DAAM1 FH2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-09-25 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 69.213, 91.895, 97.681 |
| Unit cell angles | 98.12, 90.32, 104.79 |
Refinement procedure
| Resolution | 29.820 - 2.800 |
| R-factor | 0.225 |
| Rwork | 0.225 |
| R-free | 0.28900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.300 |
| Data reduction software | DENZO |
| Phasing software | SHARP |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 49227 | |
| <I/σ(I)> | 16.5 | 1.9 |
| Completeness [%] | 87.0 | 66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | PEG 4000, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
