2Z6D
Crystal structure of LOV1 domain of phototropin2 from Arabidopsis thaliana
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2006-07-07 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.520, 66.510, 56.690 |
| Unit cell angles | 90.00, 92.42, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.18202 |
| Rwork | 0.180 |
| R-free | 0.22682 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1n9l |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.567 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | CNS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.066 | 0.136 |
| Number of reflections | 15677 | |
| <I/σ(I)> | 14.7 | 6.59 |
| Completeness [%] | 99.5 | 99.2 |
| Redundancy | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | PEG4000, Sodium acetate, pH7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
