2Z6C
Crystal structure of LOV1 domain of phototropin1 from Arabidopsis thaliana
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44B2 |
Synchrotron site | SPring-8 |
Beamline | BL44B2 |
Detector technology | CCD |
Collection date | 2007-06-22 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 61.150, 64.920, 70.810 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.100 |
R-factor | 0.19169 |
Rwork | 0.189 |
R-free | 0.24278 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1n9l |
RMSD bond length | 0.018 |
RMSD bond angle | 1.725 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.170 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.057 | 0.352 |
Number of reflections | 16967 | |
<I/σ(I)> | 41.8 | 4.8 |
Completeness [%] | 99.9 | 99.2 |
Redundancy | 13.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | ammonium sulfate, pH8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |