2Z69
Crystal Structure of the sensor domain of the transcriptional regulator DNR from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-02-09 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0723 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 56.145, 105.534, 74.838 |
| Unit cell angles | 90.00, 97.97, 90.00 |
Refinement procedure
| Resolution | 74.120 - 2.100 |
| R-factor | 0.2174 |
| Rwork | 0.215 |
| R-free | 0.26235 |
| Structure solution method | SAD, MOLECULAR REPLACEMENT |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.473 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 74.120 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Rmerge | 0.079 | 0.403 |
| Number of reflections | 23925 | |
| <I/σ(I)> | 11.2 | 6.7 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.9 | 11.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.4 | 294 | 14% (w/v) PEG 4000, 100mM Sodium Citrate (pH 5.4), 200mM Potassium Fluoride, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
