2Z68
Crystal Structure Of An Artificial Metalloprotein: Cr[N-salicylidene-4-amino-3-hydroxyhydrocinnamic acid]/Wild Type Heme oxygenase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 180 |
Detector technology | CCD |
Collection date | 2005-12-18 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 40.656, 63.057, 77.964 |
Unit cell angles | 90.00, 96.88, 90.00 |
Refinement procedure
Resolution | 29.770 - 1.580 |
R-factor | 0.18049 |
Rwork | 0.178 |
R-free | 0.22493 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wzd |
RMSD bond length | 0.013 |
RMSD bond angle | 1.474 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | X-PLOR |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.640 |
High resolution limit [Å] | 1.580 | 1.580 |
Rmerge | 0.061 | |
Number of reflections | 53304 | |
<I/σ(I)> | 23.2 | |
Completeness [%] | 99.9 | 100 |
Redundancy | 3.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES pH6.5, 0.2mM Ammonium sulfate, 30% w/w PEG 2000 methyl mono ester, VAPOR DIFFUSION, HANGING DROP, temperature 298K |