2Z5W
tBclA, a recombinant spore surface protein from Bacillus anthracis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-6A |
Synchrotron site | Photon Factory |
Beamline | BL-6A |
Temperature [K] | 138 |
Detector technology | CCD |
Collection date | 2006-06-22 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.96426 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 68.144, 68.144, 163.178 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 47.820 - 1.350 |
R-factor | 0.17485 |
Rwork | 0.174 |
R-free | 0.19107 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1WCK |
RMSD bond length | 0.010 |
RMSD bond angle | 1.407 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 81.650 | 1.380 |
High resolution limit [Å] | 1.350 | 1.350 |
Rmerge | 0.035 | 0.850 |
Number of reflections | 43616 | |
<I/σ(I)> | 74.9 | 1.1 |
Redundancy | 33 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.1M MES (pH 6.5), 17.5% EtOH, 5% PEG 400, VAPOR DIFFUSION, HANGING DROP, temperature 298K |