2Z5P
Apo-Fr with low content of Pd ions
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-03-03 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.5086 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 180.790, 180.790, 180.790 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.790 - 1.650 |
| R-factor | 0.16959 |
| Rwork | 0.169 |
| R-free | 0.18788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dat |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.144 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.693 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.085 | 0.298 |
| Number of reflections | 30995 | |
| <I/σ(I)> | 40 | 14.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 21.5 | 22.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate, Cadmium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | Ammonium sulfate, Cadmium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
