2Z5N

Complex of Transportin 1 with hnRNP D NLS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-09
Detector	ADSC QUANTUM 315
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	69.683, 118.954, 151.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.220 - 3.200
R-factor	0.23493
Rwork	0.232
R-free	0.29525
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2z5o
RMSD bond length	0.008
RMSD bond angle	1.121
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.310
High resolution limit [Å]	3.200	3.200
Rmerge	0.082	0.541
Number of reflections	18626
<I/σ(I)>	10.2	1.7
Completeness [%]	91.9	56.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.8	293	0.1M Tris-HCl pH 8.8, 16-20%(w/v) PEG 8000, 0.1M KCl, 0.1M NaH2PO4, VAPOR DIFFUSION, HANGING DROP, temperature 293K