2Z5M
Complex of Transportin 1 with TAP NLS, crystal form 2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-5A |
| Synchrotron site | Photon Factory |
| Beamline | BL-5A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-24 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 132.442, 170.986, 68.340 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.000 |
| R-factor | 0.24899 |
| Rwork | 0.248 |
| R-free | 0.26741 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2z5o |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.113 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.058 | 0.402 |
| Number of reflections | 28901 | |
| <I/σ(I)> | 14.9 | 3.8 |
| Completeness [%] | 95.2 | 71.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.8 | 293 | 100mM Tris-HCl pH 8.8, 16-18% (w/v) PEG 8000, 0.1M NaH2PO4, 0.1M KCl, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
