2Z55
Bacterioruberin in the trimeric structure of archaerhodopsin-2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44B2 |
| Synchrotron site | SPring-8 |
| Beamline | BL44B2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 63 |
| Unit cell lengths | 108.830, 108.830, 220.710 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.500 |
| R-factor | 0.201 |
| Rwork | 0.201 |
| R-free | 0.23200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ei4 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.322 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.000 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.086 | 0.420 |
| Number of reflections | 51050 | |
| <I/σ(I)> | 22.5 | 5.1 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 8.6 | 8.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 288 | 2.9M ammonium sulfate, 3.25mg/ml nonyl glucoside, 0.16M NaCl, 8% trehalose, 0.04% Na-azide, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 288K |
