2Z53

Crystal structure of the S211A mutant of the ribosome inactivating protein PDL4 from P. dioica leaves

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7A
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7A
Temperature [K]	100
Detector technology	CCD
Collection date	2006-04-08
Detector	MAR CCD 165 mm
Spacegroup name	P 21 21 21
Unit cell lengths	43.619, 61.541, 91.626
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.290
Rwork	0.138
R-free	0.16000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qcg
RMSD bond length	0.022
RMSD bond angle	0.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	il (Milione)
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	1.290	1.290
Number of reflections	61843

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION			VAPOR DIFFUSION