2Z51
Crystal structure of Arabidopsis CnfU involved in iron-sulfur cluster biosynthesis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-12-17 |
Detector | Bruker DIP-6040 |
Wavelength(s) | 0.9 |
Spacegroup name | P 2 2 2 |
Unit cell lengths | 43.546, 57.394, 61.303 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 61.310 - 1.350 |
R-factor | 0.13671 |
Rwork | 0.135 |
R-free | 0.17338 |
Structure solution method | SIRAS |
RMSD bond length | 0.030 |
RMSD bond angle | 1.634 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHARP |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 61.310 | 1.383 |
High resolution limit [Å] | 1.350 | 1.348 |
Number of reflections | 34580 | |
Completeness [%] | 99.9 | 97.97 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | PEG 1000, MgCl2, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |