2Z4U
Crystal structure of wild type PD-L4 from Phytolacca dioica leaves
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
Synchrotron site | EMBL/DESY, HAMBURG |
Beamline | BW7A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-08-20 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.9 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 43.521, 58.813, 98.774 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.100 |
Rwork | 0.122 |
R-free | 0.14600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qcg |
RMSD bond length | 0.017 |
RMSD bond angle | 0.030 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | Il (Milione) |
Refinement software | SHELXL-97 |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.200 |
High resolution limit [Å] | 1.100 | 1.100 |
Number of reflections | 102812 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 298 | 20% PEG4000, 10% isopropanol, 0.1M Hepes, pH 7.5, VAPOR DIFFUSION, temperature 298K |